Composite
Part:BBa_K748006:Design
Designed by: Lei Qiao Group: iGEM12_HIT-Harbin (2012-09-15)
S.aureus killing device. Truncated lysostaphin coding sequence with IPTG inducible promoter.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 583
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
Source
de novo synthesis